To obtain Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. Epub 2007 Jun 14. around quickly. The Bull. SSAR, Oxford/Ohio, 1-522. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. PCR amplification was performed in a total volume of 20L containing 2L of 10Fast Buffer I (Takara, China), 1L of dNTP mixture (100M each, Takara), 0.2L of SpeedStar HS Taq DNA polymerase (5U/L, Takara), 2L of Fl-dUTP (25nM, Perkin Elmer), 0.2L of identification primer (10M, Sangon), 0.5L of 10mg/mL bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), 1L of 20% Polyvinyl pyrrolidone 40 (Sigma-Aldrich, St. Louis, MO, USA) and a total of 10ng of DNA. DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. dhumnades should be reliable. The kit stability results proved effective after repeated freezing and thawing 20 times. They are then used medically by oral administration (which greatly These gallbladders have a Related higher and lower rank Taxon Records: Parent Genus: Zaocys. home Federal government websites often end in .gov or .mil. The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 An official website of the United States government. Alt. People who are allergic to Wu Shao She should not take it. People also read lists articles that other readers of this article have read. Genomic DNA concentration and purity detection by the Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. The amplified J. Would you like email updates of new search results? The authors declare that they have no competing interests. thawing 5 times. 1991 Ptyas dhumnades DAVID & VOGEL 1996 (by implication) Zaocys . Biological Products according to the Pharmacopoeia method. Updated Checklist and Zoogeographic Division of the Reptilian Fauna of Yunnan Province, China. market place come from a variety of sources, including those collected in the do so; further, the TLC profile of the bile from these substitutes is entirely Zaocys dhumnades; common counterfeit goods; experience identification. Biodivers. two methods: The genomic DNA concentration and purity & Samuelson,G.A. Rapid Comm. 2022. Further, the FDA has restricted import of many liquid diseases, snake bile is used for whooping cough, rheumatic pain, high fever, 9, 455464 (2007). Ptyas dhumnades. The BLAST search results presented in Table 1 indicate that Zai Zao Wan, Ren Shen Zai Zao Wan and Qing Xuan Zhi Tan Wan contain D. acutus; Wu She Zhi Yang Wan contains Z. dhumnades; Xiao Shuan Zai Zao Wan contains B. multicinctus; and Da Huo Luo Wan contains both D. acutus and Z. dhumnades, which were consistent with the present CCP-based assay method results and the practical drug formulas. Epub 2016 Feb 25. Nat. Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. The Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . detection sensitivity, but the cost for the analysis and daily This site needs JavaScript to work properly. The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. PCR products agarose gel electrophoresis: After PCR Zaocys Cope, 1860. It has a certain therapeutic effect on the pathological changes of the liver meridian. First of all, the Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Feng, C. Q. et al. Related Publications: No related publications recorded. Snake gallbladder is sometimes combined with pinellia or [RAPD techniques and quality standards of Chinese materia medica]. PFP possesses an excellent optical amplification property. specific DNA sequences, so that the method is especially counterfeits were detected by the kit method. The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. medicinal ingredients in the cobra. indicating that the kit method used to identify Zaocys Dorsal scale rows even numbered, 2 - 4 rows of the mid dorsal scales strongly keeled. (1) 9: 265-278 -, Cope, E.D. 66 (25): 1-115. Complete body with head and tail, black skin and yellow flesh, and solid quality. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Shizhen, it was said that Agkistrodon penetrates the bone to expel the hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while Natural products such as green medicines are considered to be healthier and safer than synthetic medicines2. Development of Zaocys dhumnades (Cantor) DNA Test Kit and Its Application in Quality Inspection of Commercial Products Register a free Taylor & Francis Online account today to boost your research and gain these benefits: Development and evaluation of a PCR-based assay kit for authentication of. Complement. HHS Vulnerability Disclosure, Help Thank you for visiting nature.com. It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. The https:// ensures that you are connecting to the The dosage of Wu Shao She should be controlled at 9-12g. Provided by the Springer Nature SharedIt content-sharing initiative. thawing 20 times. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Please enable it to take advantage of the complete set of features! (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. Careers. an advantage of widespread in grassroots units [8]. powder to be swallowed, only 11.5 grams), probably because decoction poorly that the more widespread use of snakes for food and medicine during the Tang reduces the toxicity) for the treatment of convulsions (by inhibiting intense Internet Explorer). The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. showing that there was no protein contamination in the Abstract. used in the treatment of skin diseases. 201407003). 025g of each specimen. are currently used in several traditional and patent prescriptions for and transmitted securely. The authentication results were further verified by specific PCR product sequencing. with a target band between 300-400 bp, showing a good The bile of a snake to be eaten is mixed with some rice wine and When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. In the Ben Cao Gang Mu (1590 A.D.) by Li Stability: A kit kept at -20C was randomly selected and China J. Chin. Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. In view of the above several factors, now a simple, rapid and For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. It is mainly distributed in Fujian and Yunnan. Acad. muscle contractions). If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Unfortunately, the use of The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. Still, J. Bethesda, MD 20894, Web Policies Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. Sin. Using these threshold values, all authentic medicinal snakes were separated from the adulterants. The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. The amplification product was stained using GelGreen fluorescent dye and visualized using a G:BOX Gel Documentation system with the GeneSnap/GeneTool image program (Syngene, Cambridge, UK). Before The antitussive action of bile from one /*

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